Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/14568
Title: Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks
Authors: Ferreira, Renata Trotta Barroso
Melandre, Aline Martins
Cabral, Maria Luiza
Branquinho, Maria Regina
Leite, Paola Cardarelli
Affilliation: Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.
Abstract: INTRODUCTION: Before 2004, the occurrence of acute Chagas disease (ACD) by oral transmission associated with food was scarcely known or investigated. Originally sporadic and circumstantial, ACD occurrences have now become frequent in the Amazon region, with recently related outbreaks spreading to several Brazilian states. These cases are associated with the consumption of açai juice by waste reservoir animals or insect vectors infected with Trypanosoma cruzi in endemic areas. Although guidelines for processing the fruit to minimize contamination through microorganisms and parasites exist, açai-based products must be assessed for quality, for which the demand for appropriate methodologies must be met. METHODS: Dilutions ranging from 5 to 1,000 T. cruzi CL Brener cells were mixed with 2mL of acai juice. Four Extraction of T. cruzi DNA methods were used on the fruit, and the cetyltrimethyl ammonium bromide (CTAB) method was selected according to JRC, 2005. RESULTS: DNA extraction by the CTAB method yielded satisfactory results with regard to purity and concentration for use in PCR. Overall, the methods employed proved that not only extraction efficiency but also high sensitivity in amplification was important. CONCLUSIONS: The method for T. cruzi detection in food is a powerful tool in the epidemiological investigation of outbreaks as it turns epidemiological evidence into supporting data that serve to confirm T. cruzi infection in the foods. It also facilitates food quality control and assessment of good manufacturing practices involving acai-based products.
Keywords: Chagas Disease
Trypanosoma cruzi
Euterpe
PCR detection
Food analysis
keywords: Doença de Chagas
Trypanosoma cruzi
Açaí
Detecção de PCR
Análise de Alimentos
DeCS: Doença de Chagas
Trypanosoma cruzi
Euterpe
Reação em Cadeia da Polimerase
Análise de Alimentos
Issue Date: 2016
Publisher: Sociedade Brasileira de Medicina Tropical
Citation: FERREIRA, R. T. B. et al. Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks. Rev. Soc. Bras. Med. Trop., Uberaba, v. 49, n. 2, p. 190-195, 2016.
DOI: http://dx.doi.org/10.1590/0037-8682-0414-2015
ISSN: 0037-8682
Copyright: open access
Appears in Collections:INCQS - Artigos de Periódicos

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