Title:	Serotypes and Pulsotypes Diversity of Listeria monocytogenes in a Beef-Processing Environment
Authors:	Camargo, Anderson Carlos Dias, Mariane Rezende Cossi, Marcus Vinícius Coutinho Lanna, Frederico Germano Piscitelli Alvarenga Cavicchioli, Valéria Quintana Vallim, Deyse Christina Pinto, Paulo Sérgio de Arruda Hofer, Ernesto Nero, Luís Augusto
Affilliation:	Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil. Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil. undação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil. Universidade Federal de Viçosa. Departamento de Veterinária. Viçosa, MG, Brasil.
Abstract:	Utensils and equipment from meat-processing facilities are considered relevant cross-contamination points of Listeria monocytogenes to foods, demanding tracking studies to identify their specific origins, and predict proper control. The present study aimed to detect L. monocytogenes in a beef-processing facility, investigating the diversity of serotypes and pulsotypes in order to identify the possible contamination routes. Surface samples from knives (n = 26), tables (n = 78), and employees hands (n = 74) were collected before and during the procedures from a beef-processing facility, in addition to surface samples of end cuts: round (n = 32), loin (n = 30), and chuck (n = 32). All samples were subjected to L. monocytogenes screening according ISO 11.290- 1, and the obtained isolates were subjected to serotyping and pulsed-field gel electrophoresis. Listeria spp. were identified in all processing steps, in 61 samples, and L. monocytogenes was detected in 17 samples, not being found only in knives. Eighty-five isolates were identified as L. monocytogenes, from serotypes 1/2c (n = 65), 4b (n = 13), and 1/2b (n = 7), being grouped in 19 pulsotypes. Considering these results, cross-contamination among hands, tables, and beef cuts could be identified. The obtained data indicated the relevance of crosscontamination in the beef-processing facility, and the occurrence of serotypes 1/2b and 4b in beef cuts distributed for retail sale is a public health concern.
Keywords:	Listeria monocytogenes Listeriosis Cross-contamination Foodborne diseases
DeCS:	Doenças Transmitidas por Alimentos Listeria monocytogenes Listeriose Contaminação de Alimentos
Issue Date:	2015
Publisher:	Mary Ann Liebert, Inc.
Citation:	CAMARGO, Anderson Carlos; et al. Serotypes and Pulsotypes Diversity of Listeria monocytogenes in a Beef-Processing Environment. Foodborne Pathogens and Disease, v.12, n.4, p.323-326, Apr. 2015.
DOI:	10.1089/fpd.2014.1875
ISSN:	1535-3141
Copyright:	restricted access
Appears in Collections:	IOC - Artigos de Periódicos

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